| INOS

Nitric Oxide Synthase 2, Inducible，also known as NOS2，is an enzyme encoded by the NOS2 gene. NOS2 contains a C-terminal reductase domain, which binds NADPH and transfers electrons from NADPH to FAD and then to FMN, and an N-terminal oxygenase domain, which contains a heme and binding sites for arginine, tetrahydrobiopterin and calmodulin. Nitric oxide is a reactive free radical mediating in neurotransmission, antimicrobial and antitumoral activities. Nos2 is important for protective immunity against CMV.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (IU/mL)	500	250	125	62.5	31.2	15.6	7.8	0
OD	2.67	2.05	1.48	0.97	0.67	0.42	0.27	0.09
Corrected OD	2.58	1.96	1.39	0.88	0.58	0.33	0.18	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse INOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-103	98
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse INOS and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	90-110
	Average (%)	91	96
1 : 4	Range (%)	89-105	104-115
	Average (%)	95	108